The glycoproteome remains severely understudied because of significant analytical
challenges associated with glycoproteomics, the system-wide analysis of intact
glycopeptides. This review introduces important structural aspects of protein N-glycosylation
and summarizes the latest technological developments and applications in LC-MS/MS-
based qualitative and quantitative N-glycoproteomics. These maturing technologies provide
unique structural insights into the N-glycoproteome and its synthesis and regulation by …